Development of antibody and protein purification!
Biopharmaceuticals such as antibodies and proteins have become the new backbone of the pharmaceutical industry. This document is a guide for developing IEX purification processes for antibodies and proteins with the considerations of economic and productive aspects. This means: maximum sample recovery in the shortest cycle time within cost limitations.
Contents of the whitepaper
Within this whitepaper, the important steps in method development for
antibody/protein purifications using IEX chromatography are demonstrated on a real-life example:
- Separation modes
- Selection of IEX Resin types
- Buffer composition and pH
- Definition of elution conditions
- Gradient optimisation
- Cleaning strategies
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