Development of antibody and protein purification

Whitepaper for

Development of antibody and protein purification!

Biopharmaceuticals such as antibodies and proteins have become the new backbone of the pharmaceutical industry. This document is a guide for developing IEX purification processes for antibodies and proteins with the considerations of economic and productive aspects. This means: maximum sample recovery in the shortest cycle time within cost limitations.

Contents of the whitepaper

Within this whitepaper, the important steps in method development for
antibody/protein purifications using IEX chromatography are demonstrated on a real-life example:


  • Separation modes
  • Selection of IEX Resin types
  • Buffer composition and pH
  • Definition of elution conditions
  • Gradient optimisation
  • Cleaning strategies

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